Abstract
Sclerotium rolfsii has been considered one of the most prevalent tropical sugarbeet (Beta vulgaris L. spp. Vulgaris var. altissima) pathogens in Tamil Nadu and India. Five isolates from tropical sugarbeet roots were collected in different regions and analysed through RFLP for genetic diversity. Genomic DNA was isolated from all the strains of S. rolfsii and the ITS region, which includes the internal transcribed spacer regions 1 and 2; the 5.8S rRNA genes were amplified with primers ITS1 and ITS 4. PCR amplification of the ITS regions of the S. rolfsii isolates from tropical sugarbeet gave products of approximately 700 bp in length with slight variations in a few isolates. Results showed that more divergent isolates originated from areas with a single crop. Restriction fragment length polymorphism analysis of the ITS regions of nuclear rDNA of S. rolfsii with HhaI, PstI and MspI confirmed the heterogeneity among the S. rolfsii isolates from tropical sugarbeat.
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