Molecular typing of the red-tide dinoflagellate Gonyaulax polyedra in phytoplankton suspensions

Abstract

AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials AME 09:55-61 (1995) - DOI: https://doi.org/10.3354/ame009055 Molecular typing of the red-tide dinoflagellate Gonyaulax polyedra in phytoplankton suspensions Rollo F, Sassaroli S, Boni L, Marota I A group of closely related DNA sequences each approx. 240 base pairs long ('Gp240 family') was detected in the DNA extracted from cultured cells of the red-tide dinoflagellate Gonyaulax polyedra using the polymerase chain reaction. While members of the Gp240 family appeared to be present in the DNA of all the isolates of G. polyedra tested, no related sequence could be detected in the DNA of other dinoflagellates, diatoms or marine bacteria. In a further set of experiments, the PCR system based on the Gp240 DNA was used to probe phytoplankton samples collected in the Northern Adriatic Sea during a red tide caused by G. polyedra. The results showed that the members of the Gp240 family contain a short simple-sequence stretch giving rise to length polymorphism. The potential of this molecular identification system to track G. polyedra in its natural environment is discussed. Considerations are also made on the evolution of the simple-sequence loci in marine organisms. Gonyaulax polyedra . 'Gp240 family' . Simple-sequence loci . Dinoflagellate Full text in pdf format PreviousNextExport citation RSS - Facebook - Tweet - linkedIn Cited by Published in AME Vol. 09, No. 1. Publication date: April 28, 1995 Print ISSN: 0948-3055; Online ISSN: 1616-1564 Copyright © 1995 Inter-Research.