Abstract
Background: Salmonella is one of the most widespread zoonotic enter pathogenic microorganisms found in the global food chain. Poultryand Poultry products have been identified as one of the important foodborne sources of Salmonella. Pulsed-Field Gel Electrophoresis (PFGE) is a gold standard typing method for identification of Salmonella isolates during outbreaks and epidemiological investigations. Objectives: The aim of this study was to carry out molecular typing of Salmonella enterica spp. by PFGE technique. Materials and Methods: All 47 Salmonella isolates were serotyped and then subjected to PFGE. Total isolates were analyzed by means of the molecular technique XbaI PFGE. Results: In the current work, PFGE and serotyping were used to subtype 47 Salmonella isolates belonging to 22 different serotypes and derived from poultry. Thirty-nine PFGE patterns out of 47 isolates were obtained. The Discrimination Index (DI) by serotyping (0.93) was lower than PFGE (DI = 0.99). Conclusions: In conclusion, molecular methods such as PFGE can be used for epidemiological characterization of Salmonella serotypes.
Highlights
Salmonella is one of the most widespread zoonotic enter pathogenic microorganisms found in the global food chain
Some molecular typing methods have been used for epidemiological studies of Salmonella serotypes in Iran [20,21,22], only a few recently published reports involve the use of Pulsed-Field Gel Electrophoresis (PFGE) for characterization of Salmonella serotypes in poultry in Iran [5, 23]
Non-typhoidal Salmonella contamination in poultry is a major problem in Asian countries as well as the other countries, in terms of both morbidity and economic costs [7, 27]. Most of these infections have been attributed to the consumption of poultry meat and eggs [28, 29]
Summary
Salmonella is one of the most widespread zoonotic enter pathogenic microorganisms found in the global food chain. Pulsed-Field Gel Electrophoresis (PFGE) is a gold standard typing method for identification of Salmonella isolates during outbreaks and epidemiological investigations. Conclusions: In conclusion, molecular methods such as PFGE can be used for epidemiological characterization of Salmonella serotypes. Molecular typing methods such as Random Amplified Polymorphic DNA-PCR (RAPD-PCR), Repetitive -PCR (Rep-PCR), Enter bacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR), Pulsed Field Gel Electrophoresis (PFGE), and Amplified Fragment Length Polymorphism (AFLP) have been developed for characterization of Salmonella serotypes [5, 14, 15]. Some molecular typing methods have been used for epidemiological studies of Salmonella serotypes in Iran [20,21,22], only a few recently published reports involve the use of PFGE for characterization of Salmonella serotypes in poultry in Iran [5, 23]
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