Molecular typing of Brucella abortus strains in Xinjiang

Abstract

Objective To identify molecular typing of Brucella abortus isolates in Xinjiang, and determine the identification ability of multiple locus variable-number tandem repeat analysis (MLVA). Methods The optimized Brucella AMOS-PCR was used for identification of Brucella(n= 7) genus and species in Xinjiang from 2010- 2015, and MLVA-16 was used to further identify the isolates. Results were compared with the data of the Brucella standard strain provided by the http://mlva.u-psud.fr database. Cluster analysis was carried out with Bionumerics 6.6. Results The results of AMOS-PCR and MLVA-16 were identical, all were Brucella abortus. Further classification results of the MLVA-16 showed that the strain in Xinjiang was type 3 of Brucella abortus, which was basically the same as that of the domestic Brucella. Conclusions The molecular typing of isolates separated in Xinjiang is type 3 of Brucella abortus. MLVA can identify Brucella at the level of species, and highly sensitive to Brucella biotype and isolates differences, which provides a basis for the traceability and evolution of brucellosis epidemic strains. Key words: Brucella; Biological assay; Bacterial typing techniques