Abstract
BackgroundAcinetobacter baumannii is an opportunistic pathogen that can cause several kinds of nosocomial infections. Increasing antibiotic resistance as well as identifying genetic diversity and factors associated with pathogenicity and prevalence of this bacterium is important. The aim of this study was the investigation of molecular typing, biofilm production, and detection of carbapenemase genes in multidrug-resistant Acinetobacter baumannii isolated from different infection sites using ERIC-PCR in Iran.MethodsForty isolates of A. baumannii were obtained from various wards of the central hospital, in the west of Iran. Phenotypic identification and genetic diversity, biofilm production assay, and detection of Carbapenemase genes carried out.ResultsTracheal samples 26 (61.9 %) are the most frequent isolates, and 95 % of isolates were identified as MDR. 32.5 % of all A. baumannii strains were capable to form a strong biofilm. It was founded that antimicrobial resistance patterns had a significant relationship with strong biofilm formation (P = 0.001). Most frequencies of the studied genes were in the order of VIM (81 %), SPM (45.2 %), and IMP (35.7 %) genes. The VIM gene was the most frequent in all isolates which were significant (P = 0.006). 14 different ERIC-types were observed including 7 common types and 7 unique or single types. F type is the largest common type consisting of nine isolates and B, D, and E types contain two isolates separately.ConclusionsERIC-PCR technique was used to genetically classify A. baumannii isolates as one of the most common microorganisms in nosocomial infections.
Highlights
Acinetobacter baumannii is an opportunistic pathogen that can cause several kinds of nosocomial infections
The purpose of this study is to investigate the relationship between molecular typing, biofilm formation, and detection of carbapenemase genes in multidrug-resistant A. baumannii isolated from different infections in Hamadan, Iran
According to antibiotic susceptibility testing, 38 out of 40 (95 %) A. baumannii isolates were identified as MDR
Summary
Acinetobacter baumannii is an opportunistic pathogen that can cause several kinds of nosocomial infections, including surgical site infections, skin and soft tissue infections, urinary tract infections (UTI), ventilator-and nosocomial‐associated pneumonia, catheter-related bloodstream infections, and secondary meningitis [1, 2]. This bacterium is unique due to enabling it to survive in a variety of conditions for long periods and to colonize on the surfaces and materials in hospitals, enabling its transfer between patients through human reservoirs or inanimate elements [3, 4]. Many studies have been emphasized the biofilm formation in A. baumannii, because microbes that present in the biofilm structure have less sensitivity to antimicrobials and are more resistant to environmental situation surfaces such as catheters, intubation tubes, and cleaning types of equipment, which indicates the critical role of biofilm in the treatment failure and increase hospitalization time [9, 10]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
