Abstract
Spine gourd ( Momordica dioica Roxb . ) is an important vegetable with high food value and sex specific versatile medicinal value . M omordica dioica , a perennial, rhizomatous, distinctly dioecious climber belongs to family cucurbitaceae. As there are no distinguished morphological markers to identify sex, an easy, rapid and reliable molecular method for female plant identification at pr e - flowering stage in Momordica dioica is reported in this paper. Molecular tool like Random amplification of polymorphic DNA was used to identify female pla nts before pre - flowering stage. A total of 50 random decamer primers were used for screening of spec ific Random amplification of polymorphic DNA markers in male and female populations. Only one primer OPA - 15 amplified genomic DNA in different patterns in male and female genotypes. This sex specific band OPA - 15 900 was identified only in female lines but n ot in male lines. This marker may be efficiently used as effective, convenient and reliable molecular markers for female identification in Momordica dioica at pre - flowering stages so that it can be cultivated and utilized for its medicinal purpose. Keyword s: Momordica dioica , Sex determination , Molecular tool, RAPD, SCAR marker .
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