Abstract
AbstractWith saline water as the continuous medium, a two‐component McMillan‐Mayer equation of state is used to describe liquid–liquid phase equilibria in a two‐protein system. The equation of state is based on a hard‐sphere reference with perturbations introduced through a potential of mean force to account for electrostatic forces and for attraction between protein particles. To illustrate the thermodynamic framework, one parameter each is fitted to experimental precipitation data for aqueous saline one‐protein systems containing either lysozyme or ovalbumin. A lysozyme–ovalbumin interaction parameter is then introduced to calculate phase behavior in the aqueous two‐protein system. These calculations are remarkably similar to classic vapor–liquid equilibrium calculations using an equation of state. For the aqueous two‐protein system, calculations give the light‐phase composition as well as the lysozyme and ovalbumin partition coefficients for a given dense‐phase composition. Agreement with sparse experimental data is reasonable over a range of pH and high ionic strength provided by the common precipitant ammonium sulfate.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.