Abstract
In the last two decades the application of molecular techniques has had a major impact on the classification of yeasts. The nuclear DNA relatedness has become the basis of species delineation. Molecular fingerprinting methods such as analysis of restriction fragment length polymorphisms, random amplified polymorphic DNA, PCR-amplified sequences and fragments, pulsed field gel electrophoresis of chromosome DNA and others allow intraspecies differentiation and typing. The most far reaching method has been the sequencing of various parts of ribosomal DNA that has made for the first time possible to assess the phylogenetic relationships among yeasts at different taxonomic levels. Based on the molecular data obtained so far several changes have been introduced in the classification of yeasts, however, substantial restructuring of current taxonomic schemes with the consequence of numerous nomenclatural changes must await further studies.
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