Molecular systematics of western North American cyprinids (Cypriniformes: Cyprinidae)

Abstract

The phylogenetic or evolutionary relationships of species of Cypriniformes, as well as their classification, is in a era offlux. For the first time ever, the Order, and constituent Families are being examined for relationships within aphylogenetic context. Relevant findings as to sister-group relationships are largely being inferred from analyses of bothmitochondrial and nuclear DNA sequences. Like the vast majority of Cypriniformes, due to an overall lack of anyphylogenetic investigation of these fishes since Hennig’s transformation of the discipline, changes in hypotheses ofrelationships and a natural classification of the species should not be of surprise to anyone. Basically, for most taxa noproperly supported phylogenetic hypothesis has ever been done; and this includes relationships with reasonable taxonand character sampling of even families and subfamilies. As such, like others, many western North American cyprinidgenera have had a controversial taxonomic and systematic history.Our effort to better understand the evolutionary history of this artificial geographic grouping of species (Western)surveyed taxa and characters broadly. We analyzed 127 taxa (71 species) from 36 genera, including representative taxafrom all 22 western genera hypothesized to form the Western Clade sensu Coburn and Cavender (1992). Our evaluationalso included additional sampling from a heterogeneous array of species from the western genera Algansea, Gila,Lepidomeda, Ptychocheilus and Siphateles. Resulting phylogenetic inferences, based on one mitochondrial and threenuclear genes (mtDNA: cytb; nDNA: Rag1, Rhod, S7), consistently resolved a well-supported Western Clade, but oneinclusive of Chrosomus erythrogaster. This taxon, always formed the sister group to the extant species of Gila plus 10other western genera. Our Western Clade is qualitatively different from that of prior studies and does not include thegenera Agosia, Algansea, Iotichthys, Lepidomeda, Meda, Mylocheilus, Plagopterus, Pogonichthys, Rhinichthys, Tiarogaor Yuriria. All of these taxa were, however, included in Coburn and Cavender´s (1992) Western Clade. Our broader-scalesurvey and increased character sampling were always resolved these latter taxa within one of two different major clades:the OPM Clade (sensu Mayden 1989) and the Creek Chub—Plagopterin Clade (sensu Simons et al. 2003). Ourhypothesized Western Clade places Orthodon sister to a Western Chub-Pikeminnow Clade also inclusive of Acrocheilus,Eremichthys, Gila, Hesperoleucus, Lavinia, Moapa, Mylopharodon, Ptychocheilus, Relictus and Siphateles. The lattertaxa have traditionally been recognized at the generic level, simply on the basis of their morphological distinctivenessand not on the basis of a phylogenetic evaluation of relationships. Composition of our Western Chub-Pikeminnow Cladealso reveals genetic divergences between species of some genera (Gila, Ptychocheilus, Siphateles) comparable to geneticdivergences documented between genera within the Western Clade. Relationships for these 10 genera also highlighttaxonomic inconsistencies relative to recent phylogenetic analysis and, in some cases, are in need of focused attentionusing morphology or additional molecular data to test relationships that will eventually establish a stable classification.Some of these genera are clearly unnatural relative to other genera and their classification or ranking is an obligatory change in modern science of phylogenetics.