Abstract
Plastid trnL-trnF and nuclear ribosomal ITS sequences were obtained from selected wild-type individuals of Polygonum minus Huds. in Peninsular Malaysia. The 380 bp trnL-trnF sequences of the Polygonum minus accessions were identical. Therefore, the trnL-trnF failed to distinguish between the Polygonum minus accessions. However, the divergence of ITS sequences (650 bp) among the Polygonum minus accessions was 1%, indicating that these accessions could be distinguished by the ITS sequences. A phylogenetic relationship based on the ITS sequences was inferred using neighbor-joining, maximum parsimony and Bayesian inference. All of the tree topologies indicated that Polygonum minus from Peninsular Malaysia is unique and different from the synonymous Persicaria minor (Huds.) Opiz and Polygonum kawagoeanum Makino.
Highlights
Current advances in plant molecular biology techniques and approaches provide a convenient and rapid evaluation of the differences in informational content, derived from DNA sequence data, of related individuals [1,2]
In this study, we aim to highlight the potential impact of molecular genetic marker on selected P. minus accessions from Peninsular Malaysia based on the trnL-trnF and nuclear ribosomal ITS sequences in order to clarify the taxonomic status of P. kawagoeanum and
The trnL-trnF sequences among the four P. minus accessions were identical. This result implies that the trnL-trnF region from the plastid genome was not suitable to differentiate individuals of P. minus
Summary
Current advances in plant molecular biology techniques and approaches provide a convenient and rapid evaluation of the differences in informational content, derived from DNA sequence data, of related individuals [1,2]. Polymerase chain reaction (PCR)-based techniques have been used comprehensively as plant molecular markers for significant phylogenetic presumption among relatively closely related species [3]. Polygonum minus Huds., commonly known as kesum in Malaysia, is currently classified in the Polygonum section Persicaria. No additional information on P. minus was available until Anjen et al [7] and Freeman and Reveal [8] reported Polygonum kawagoeanum Makino and Persicaria minor (Huds.) Opiz, respectively, as being synonyms of P. minus. For the first time, we reported peltate glandular trichomes and conical brushlike clustered trichomes on both the adaxial and abaxial surfaces of
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