Molecular surveillance of avian influenza A viruses in Basrah and Wasit

Abstract

The aim of this study was to detect influenza A virus in broiler chickens and wild ducks in different geographical regions of Basrah and Wasit provinces, Iraq. This study was authenticated by analysing the viral genome and designing a set of universal primers for the detection of all influenza A subtypes in a single enzymatic reaction through the amplification of a highly conserved region of viral M gene. A total of 157 and 155 oropharyngeal and cloacal swabs from broiler chickens and wild ducks, respectively, were analysed. The study shows that influenza A viruses were prevalent in these birds in all study regions with a significantly higher percentage in wild ducks compared to broiler chickens. The results showed that 92/157 samples (54/75 wild ducks and 38/82 broiler chickens) and 96/155 samples (38/75 wild ducks and 58/80 broiler chickens) in Basrah and Wasit, respectively, were positive for the viral M gene. In addition, there was no significant difference in virus prevalence between Basrah and Wasit provinces.