Molecular study of Toxoplasma gondii infection in normal delivery women with previous abortion history at Basrah Province

Abstract

Toxoplasma gondii is the causative agent of toxoplasmosis, one of the most common parasitic infections of humans and pets. T. gondii was serologically investigated for newborn women who had previous abortion by ELISA and included (70) women with a history of single or recurrent abortion and (30) women with normal delivery without previous abortion as a control group. The study included women attending Basrah Obstetrics and Gynecology Hospital, Al-Moanaa General Hospital, Al-Fayhaa General Hospital, Zubair General Hospital and Dar Al Shifa investment Hospital. The percentage of seroprevalence of T. gondii IgG was (41.4%). No percentage was recorded for IgM antibodies. Significant differences were found between age groups of these antibodies. The examination showed that 7 (23.3%) of (30) in the control group were positive for IgG antibodies, compared to 0 (0%) of IgM. The highest percentage (50%) of IgG antibody was in the age group 20 years or less according to the number of previous miscarriages. The highest percentage of IgG antibody infection was (46.2%) in women who had two previous abortions and no infection rates were recorded for IgM antibody. The highest infection rates (43.8%) were in Zubair district compared to the city center for IgG antibody, and no infection with IgM antibody was recorded. Infection rate (70%) was recorded for the PCR-positive samples using Nested PCR technique. Toxoplasma DNA sequences according to the isolated hosts was carried out. The reading of the genetic tree showed that the sample of women born naturally is identical with the main branch of the kinship tree that carries the sequence MZ717187, MZ717188, MZ717189, MZ717190, MZ717191 in the global gene bank.