Abstract
Background:There are scarce reports about the association ofKlebsiella oxytoca(K. oxytoca) with urinary tract infection (UTI) in children. We aimed to evaluate the prevalence offimA, mrkA, matBandpilQadhesins genes and extended-spectrum beta-lactamase (ESBL) genesblaCTX-M, blaTEMandblaSHVby polymerase chain reaction (PCR) and to study biofilm formation and antibiotics resistance inK. oxytocafrom children with UTI.Methods:This study was a retrospective cross-sectional study that included 120 children with UTI due toK. oxytoca. The bacteria were subjected to molecular detection offimA, mrkA, matBandpilQadhesins genes and ESBL genesblaCTX-M, blaTEMandblaSHVby PCR. Biofilm capacity was determined by the microtiter plate method.Results:The isolatedK. oxytocahad positive ESBL activity in 45.8% of isolates. About 40% of isolates were biofilm producers. The frequency of adhesion genes amongK. oxytocawas 91.7%, 83.3%, 48.3% and 37.5% formatB, pilQ, fimAandmrkAgenes, respectively. For ESBL genes, the frequency was 38.3%, 36.7% and 33.3% forblaCTX-M, blaSHVandblaTEMgenes, respectively. The commonest genes among ESBL isolates wereblaCTX-M(83.6%),blaSHV(80%) thenblaTEMgene (72.7%). A significant association (p=0.048) was detected between ESBL activity and biofilm formation byK. oxytoca.Conclusion:Present study highlights the emergence ofK. oxytocaas a pathogen associated with UTI in children. There was a high prevalence of adhesin genes and ESBL genes among these isolates. The capacity ofK. oxytocato form biofilm was associated with ESBL production.
Highlights
Urinary Tract Infection (UTI) is a global health problem affecting children, common among neonates and infants [1, 2]
The aims of the present study were to evaluate the prevalence of the fimA, mrkA, matB and pilQ encoding adhesins genes and extended-spectrum beta-lactamase (ESBL) genes blaCTX-M, blaTEM and blaSHV by polymerase chain reaction (PCR) and to study the biofilm formation and antibiotics resistance in K. oxytoca isolated from children with urinary tract infections
The present study highlights the emergence of Klebsiella oxytoca as a pathogen associated with urinary tract infections in children
Summary
Urinary Tract Infection (UTI) is a global health problem affecting children, common among neonates and infants [1, 2]. The infection is more common in male neonates and female children, with a global estimated incidence of UTI among girls 3–5%, while among boys, it is 1% [3]. The misuse of antibiotic therapy without available laboratory data is the leading cause of the emergence of extended-spectrum beta-lactamases (ESBL) [9] and carbapenem-resistant Enterobacteriaceae (CRE) [10]. We aimed to evaluate the prevalence of fimA, mrkA, matB and pilQ adhesins genes and extended-spectrum beta-lactamase (ESBL) genes blaCTX-M, blaTEM and blaSHV by polymerase chain reaction (PCR) and to study biofilm formation and antibiotics resistance in K. oxytoca from children with UTI
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