Molecular study of Babesia spp and Theileria spp in camels of Al- Diwaniyah province in Iraq

Zainab Hussain Chyad Al-Naily,Ghaidaa Abbas Jasim

doi:10.36326/kjvs/2018/v9i24161

Abstract

Because of the economic importance of the camels and the scarcity of studies related to it in Iraq, our study aimed to investigate Babesia spp and Theileria spp by molecular methods. The current study was conducted during the period from September 2017 to March 2018 and collected 200 random blood samples from camels included (125 females and 75 males and two groups of ages )150(>1 years) and 50)<1year ( some of them appear on it clinical signs and some did not show any symptoms from the slaughterhouse of Al- Diwaniya province. This study was designed to diagnose these parasites firstly by microscopic examination by Geimesa stain method .The results each of (106/200)(53%) Babesia spp and (93/200)(46.5%) Theileria spp. The prevalence of Babesiosis in the female (76/125)(60.8%) more than in the males (30/75)(40%) and in different ages. Theileriosis also recorded the prevalence in female (69/125)(55.2%) and in males (24/75)(32%) and in different ages. The highest incidence in months (September, October and March) (88.3%, 96.2% and 75%), respectively, according to the study time. recorded mix infection 19(23.75%) between Babesia &Theileria then diagnosis 90 blood samples positive microscopically using molecular techniques, which included (conventional PCR monoplex and multiplex) 16 (17.77%), 9(10%), 8(8.9%) and 11(12.22) Theileria spp , Theileria annulata, Babesia bovis and Babesia bigemina respectively .

Highlights

Because of the economic importance of the camels and the scarcity of studies related to it in Iraq, our study aimed to investigate Babesia spp and Theileria spp by molecular methods
Babesia spp and Theileria spp and this fact confirm that the disease is widespread and affects all animals species including camels[15]
The microscopic examination of the blood smear showed that the Babesia spp and Theileria spp appear in several shapes inside RBCs which find are agreement with [3,15]

Summary

Molecular methods microscopy and then DNA extraction

Used conventional PCR by specific for is confirm diagnosis by the use of primers for detection Babesia spp and technologist (PCR) with analysis. Theileria spp and PCR thermo cycler sequence[15]. There are no control system as following table[1]. Denatura Denatura Anneali Extensi extension d tion tion ng on Babesia bovis 94°C/280s 94°C/120s 59/60sec 72°C/60 72°C/60s 4°c ec ec sec ec Babesia. 94°C/280s 94°C/120s 58/60sec 72°C/60 72°C/60s 4°c bigemina ec ec sec ec Theileria. Specific primers provided by IDT(Canada) as( table -2)

F AGTTTCTGACCTATCA

Discussion

Findings

Methods