Molecular study for antibiotic-resistance patterns of extended-spectrum B- Lactamase producing Klebsiella Pneumoniae from a UTI of pregnant women in Diwaniyah (Shamiya city) - Iraq

Abstract

Background: Klebsiella pneumoniae is a member of the Enterobacteriaceae family. K. pneumoniae isolates produce enzymes that cause the hydrolysis of penicillins and cephalosporins.These enzymes are known as broad-spectrum beta-lactamases (ESBL). The resistance of K. pneumoniae to beta-lactamase antibiotics is usually mediated by beta-lactamase genes, and the present study aims to determine the prevalence of ESβL producing K. pneumoniae isolates with their antibiotic susceptibility pattern in urine samples of pregnant women with UTI. Method: The current study included two hundred twenty-five pregnant women as if they were suffering from urinary tract infection and were treated in the Shamiya General Hospital and the Pregnant Care Center in the Shamiya sector from October 2021 to March 2022. One hundred forty nine different types of bacteria were isolated and using diagnostic methods nineteen isolates of Klebsiella pneumoniae were obtained. Results: The results revealed that 225 pregnant women were the results were positive for 149 patients with urinary tract infections, and the bacterial growth was of different types the highest rate of infection was in the age group of 21–25 years. Then, 19 positive samples of Klebsiella pneumoniae were detected using chemical and morphological methods. Penicillin resistance was 100%.