Molecular Studies on Toxoplasma Gondii in Sheep and Goats in Ismailia Province, Egypt

Abstract

Diagnosis of Toxoplasma gondii in 20 brain samples (10 from sheep and 10 from goats) using nested PCR techniques by amplification of the T. gondii B1 gene revealed that, six sheep’s brain samples were positive (60%) and six goat’s brain samples were positive (60%). Bioassay was done by inoculation of T. gondii positive PCR samples into healthy mice. Histopathological examination of the experimental mice brains, declared pathological lesions with demonstration of pseudocysts containing bradizoites within the host cell cytoplasm in the mice killed 30th day and 35th day post inoculation.