Abstract
This study was aimed to isolate and identifyPseudomonas aeruginosa (P. aeruginosa) from 150 diseased broiler chickens in addition to 50 environmental swabs from water pipes and tanks to examine their susceptibility against some commonly used antimicrobial agents, in addition to detection of some virulence genes using Polymerase Chain Reaction (PCR) and evaluation of its ability to form biofilm in vitro. Clinically the affected chickens were subjected to postmortem examination (P.M) then samples from internal organs such as (liver, heart, lung, spleen, and intestine); tracheal and environmental swabs were collected and subjected to bacteriological examination and identification. Twenty P. aeruginosa isolates were recovered from the diseased chickens and environmental swabs with an incidence of (10%). Sixteen isolates were isolated from internal organs of 150 diseased chickens with an incidence of (10.66%); meanwhile 4 isolates were recoded from 50 water pipes and tanks swabs with a percentage of (8%). Antimicrobial sensitivity testing showed highly sensitivity to amikacin and colistin sulphate with percentages of (90%) for each of them. Doxycycline showed resistance with a percentage of (75%). PCR technique was a good tool for testing three virulence genes; pslA, pelAand fliC genes; the three genes were detected in of the examined samples with a percentage of (100%). A significant relationship between the existence of three virulence genes in the isolated P. aeruginosa and ability of biofilm formation (Slime producing ability) was reported in this study.
Highlights
P. aeruginosa is a serious poultry pathogen and zoonotic bacterial agent causing nosocomial infections (Elsayed et al, 2016); it’s associated with heavy economic losses in broiler farms (Devriese et al, 1975) causing significant morbidity and mortality (Hancock and Speert, 2000).P. aeruginosa is a motile Gram negative rod shaped bacteria belonged to family Pseudomonadaceae (Blanc et al, 1998); grows readily on common bacteriologic media and producing a water-soluble green pigment composed of fluorescein and pyocyanin (Barnes, 2003)
Sixteen isolates were isolated from internal organs of 150 diseased chickens with an incidence of (10.66%); 4 isolates were recoded from 50 water pipes and tanks swabs with a percentage of (8%)
2 - Incidence of P. aeruginosa among the examined samples A total of 20 P. aeruginosa isolates were isolated from 200 samples (150 diseased chickens and 50 water pipes and tanks swabs) with an incidence of (10 %)
Summary
P. aeruginosa is a serious poultry pathogen and zoonotic bacterial agent causing nosocomial infections (Elsayed et al, 2016); it’s associated with heavy economic losses in broiler farms (Devriese et al, 1975) causing significant morbidity and mortality (Hancock and Speert, 2000).P. aeruginosa is a motile Gram negative rod shaped bacteria belonged to family Pseudomonadaceae (Blanc et al, 1998); grows readily on common bacteriologic media and producing a water-soluble green pigment composed of fluorescein and pyocyanin (Barnes, 2003). P. aeruginosa produce a variety of toxins and enzymes that may contribute to its pathogenicity (Lin et al, 1993), birds infected with P. aeruginosa suffer from septicemia, respiratory infections, sinusitis, keratoconjunctivitis and embryonic death rates in hatcheries (Hai-ping, 2009). It produces cheesy deposits in serous surfaces lining air sacs and peritoneal cavity in addition to congestion of internal organs, perihepatitis and pericarditis (Riad, 1994)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
