Abstract
Nuclear pore complexes (NPCs) are the gateways for nucleocytoplasmic exchange. Measurements of molecular transport through NPCs may provide valuable information to unravel the mechanism of communication between the nucleus and the cytoplasm. Unfortunately, because single molecules undergo very rapid transport, it is challenging to follow their motion in live cells. We set out to address the nanomechanical basis of pore function in intact cells by a combination of fluorescence correlation spectroscopy (FCS) and real-time tracking of the center of mass of single NPCs.
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