Abstract
Molecular features of nrDNA-ITS sequences in medicinal plant Isodon rugosus were assessed with universal ITS-1 and ITS-2 primers. ITS sequences of 46 Isodon species were defined for their respective ITS-1, 5.8S and ITS-2 regions through in-silico datamining and analysis. Only in 32 species, sequence of all the three defined regions was complete, while in the rest of 14 species was partial. Isodon rugosus revealed five genic regions, namely, 18S rRNA gene (partial sequence, > 1…..38), ITS-1 (complete sequence, > 39…..236), 5.8S rRNA gene (complete sequence, > 237…..400), ITS-2 (complete sequence, > 401…..607) and large subunit 26S rRNA gene (partial sequence, > 608…..672). The sequence regions were recorded with variable sites (VS), singleton (ST) and species-specific parsimonious-informative (PI) sites. The information was also collected in a phylogenetic tree obtained using the maximum likelihood (ML) method.
Highlights
The genus name Isodon (Schrad. ex Benth.) Spach comes from the Greek word ‘isos’ means equal and ‘odous’ means tooth (Bentham, 1832; Spach, 1840)
Plant leaf material was used from the same specimen of I. rugosus (IRBSA-01), which has been studied previously by Srivastava et al (2020) in the field population of Baru-Sahib valley of Himachal Pradesh (India)
The sequence amplified by the primers 18S ITS-U1 and 26S ITS-U4 showed the overall length of amplified nrDNA-ITS regions with 569 nucleotides
Summary
The genus name Isodon (Schrad. ex Benth.) Spach comes from the Greek word ‘isos’ means equal and ‘odous’ (or odontos) means tooth (Bentham, 1832; Spach, 1840). Ex Benth.) Spach comes from the Greek word ‘isos’ means equal and ‘odous’ (or odontos) means tooth (Bentham, 1832; Spach, 1840) It consists of more than 150 species (Liu et al, 2017), distributed in tropical to temperate East Asia and tropical Africa (Mabberley, 2008; Chen et al, 2017). Are reliable, reproducible and unaffected by the physical form and environment They provide a convenient tool for identifying medicinal plants, crude drugs and even commercial products (Srivastava & Saggoo, 2014; Zhou et al, 2014). This research is in addition to our previous work in the plant I. rugosus, where the molecular autograph of plastid marker/gene (matK) was studied (Srivastava et al, 2020). Srivastava D.K., Bansal P., Singh P.K., Saggoo M.I.S
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