Molecular Screening of Salmonella sp. from fecal sample of Sparrows (Passer domesticus) in Yogyakarta, Indonesia

Marla Anggita,Okti Herawati,Sidna Artanto

doi:10.1051/bioconf/20213307003

Abstract

Wild birds is one of the reservoir agent of some of various zoonotic diseases. The study was aim to see the potential of sparrow as the reservoir agent of Salmonella sp. using polymerase chain reaction (PCR) method. We detected the invA gene of Salmonella sp. from faecal sample of sparrows (Passer domesticus) in local area of Yogyakarta, Indonesia. A total of 30 faecal dropping samples were collected from sparrows. DNA was extracted from the faecal samples, then amplified by PCR for the target genes. The amplicons were electrophorized to see the visualization of DNA on the agarose gel. The result showed the prevalence of the positive result of Salmonella sp. was 3,3%. The study indicated that sparrows can spread zoonotic pathogens and this necessitates monitoring for the epidemiologic status of these pathogens among birds, also applying the appropriate intervention measures to prevent the transmission of zoonotic diseasesfrom birds to humans.

Highlights

The current pandemic issues in the world currently has made public attention to the various possibilities of wild animals as the important objects in the spread of zoonotic diseases
Salmonella sp. detection was carried out using invA gene targeted primers forward (5’CGGTGGTTTTAAGCGTACTCTT-3’) and reverse (5’-CGAATATGCTCCACAAGGTT A-3’) with the invA gene target (769 bp)
This study reports the prevalence of Salmonella sp. in sparrow birds from wild of Yogyakarta region, Indonesia from the fecal samples

Summary

Introduction

The current pandemic issues in the world currently has made public attention to the various possibilities of wild animals as the important objects in the spread of zoonotic diseases. Several species of wild birds are known to act as reservoir for zoonotic agents such as Chlamydophila psittaci [2], Avian Influenza [3], α-, β-, γ-, and δcoronaviruses [4] enteropathogenic E.coli (EPEC) and Shiga-toxin producing E. coli (STEC) [5], and Salmonella [6]. The transmission of the diseases occurs between the wild birds to other animals and humans through droplets of feces or nasal fluid [7, 8]. Type of wild birds included in the order Passeriformes, Psittaciformes, and Columbiformes are recorded as a reservoir of various zoonotic diseases caused by bacteria [11, 12]

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Results

Conclusion