Molecular screening of Indian garlic genotypes (Allium sativum L.) for bolting using DNA based Bltm markers

Abstract

In the present study, we screened 32 Indian garlic (Alliumsativum) germplasm to identify bolting genotype using threemitochondrial DNA based molecular markers, P1, P2, andP3. In two different combinations P1+P2 and P1+P3. 1.4 kbamplicon was obtained with P1+P2 bolt marker in all the 32screened accessions which showed the inherent ability ofbolt with accessions and further needs to impose to intensewinter. Further, the consistency of result was confirmed withP1+P3 mitochondrial DNA based marker which producesthe amplicon of 3.7kb size, on the basis of nonsynchronizationin molecular profiling, the whole populationhas been classified in two groups, i.e. bolting and not boltingbased on presence or absence of both or one of them. Group-1 includes 22 (656, 662, 663, 664, 665, 667, 668, 669, 675, GG-1, GG-2, G-41, G-355, AC-50, AC-183, AC-316, SG-1, CG-1,Phule Baswant, Bhima Purple, Bhima Omkar, and AKG-2)accessions having the amplicons of both P1+P2 and P1+P3markers 1.4 and 3.7 kb respectively which indicates chimericgene arrangement. Group-2 consists of 10 accessionsshowing 1.4 kb amplicon with P1+P2 marker, includingbolting genotypes (one complete, G-5 and nine, G-3, 654,671, Ranibennur Local, Gadag Local, GG-4, G-282, AC-378,Godavari) hence it can be concluded that Indian garlicgenotypes do not need chimeric gene arrangement forbolting. This is the first step towards the identification ofshort day bolted garlic in India.