Molecular Role of SGK3 Gene in Promoting the Progression of Non-small Cell Lung Cancer

Abstract

Objective: To investigate the role of SGK3 in the progression of non-small cell lung cancer and investigate its effect on cell growth using lung cancer cell lines. Method: A retrospective analysis was conducted on 134 non-small cell lung cancer patients diagnosed in our hospital from January 2014 to December 2018, including 83 cases of adenocarcinoma and 51 cases of squamous cell carcinoma. Immunohistochemical staining was used to divide lung cancer specimens from patients into SGK3 negative and positive groups, and the correlation between SGK3 expression and clinical data was analyzed. Subsequently, cellular level experimental validation was carried out using the A549 lung cancer cell line. Result: Clinical data confirmed that SGK3 was significantly overexpressed in lung cancer tissue (P<0.05), and the difference in its expression level was not related to factors such as age and gender of SGK3 patients, but only showed a significant correlation with TNM staging and lymph node metastasis (P<0.05). In addition, through CCK-8 cell growth rate measurement experiments and cell scratch and invasion experiments, it is speculated that the above clinical findings may be due to the pro cancer effect of SGK3. Conclusion: SGK3 is highly expressed in cancer tissues of non-small cell lung cancer patients. SGK3 promotes lung cancer cell growth and division, suggesting that this regulatory factor may be involved in the occurrence and development of non-small cell lung cancer.