Abstract
The plant hormone jasmonic acid (JA) has an important role in many aspects of plant defense response and developmental process. JA triggers interaction between the F-box protein COI1 and the transcriptional repressors of the JAZ family that leads the later to proteasomal degradation. The Jas-motif of JAZs is critical for mediating the COI1 and JAZs interaction in the presence of JA. Here, by using the protoplast transient gene expression system we reported that the Jas-motif of JAZ1 was necessary and sufficient to target a foreign reporter protein for COI1-facilitated degradation. We fused the Jas-motif to the SHY2 transcriptional repressor of auxin signaling pathway to create a chimeric protein JaSHY. Interestingly, JaSHY retained the transcriptional repressor function while become degradable by the JA coreceptor COI1 in a JA-dependent fashion. Moreover, the JA-induced and COI1-facilitated degradation of JaSHY led to activation of a synthetic auxin-responsive promoter activity. These results showed that the modular components of JA signal transduction pathway can be artificially redirected to regulate auxin signaling pathway and control auxin-responsive gene expression. Our work provides a general strategy for using synthetic biology approaches to explore and design cell signaling networks to generate new cellular functions in plant systems.
Highlights
Being autotrophic and fixed in space, plants are under relentless challenges by numerous environmental stresses
Plants carry out multiple layers of response to coordinate development and defense, orchestrated by an elegantly organized signaling network of phytohormones, such as auxin that acts as a general coordinator of plant development and jasmonic acid that mainly regulates plant response to environmental stimuli [1,2,3]
jasmonic acid (JA) can directly activate the expression of genes that are involved in auxin biosynthesis to regulate tissue regeneration and lateral root formation in Arabidopsis [5,6,7]
Summary
Being autotrophic and fixed in space, plants are under relentless challenges by numerous environmental stresses. Over recent years, mounting lines of studies revealed extensive crosstalk, either synergistic or antagonistic, among different hormonal signaling pathways [4]. JA can directly activate the expression of genes that are involved in auxin biosynthesis to regulate tissue regeneration and lateral root formation in Arabidopsis [5,6,7]. On the other hand, auxin stimulates adventitious root formation on Arabidopsis hypocotyls by inducing expression of genes encoding enzymes that are involved in inactivating JA or reducing JA accumulation [8]. Elucidating hormone signaling pathways is important for understanding fundamental questions in plant biology and crucial for
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