Abstract
AbstractMaturation in bovine oocytes is triggered by a single surge of gonadotropins, i.e., luteinizing hormone (LH) and follicle‐stimulating hormone (FSH), which cause changes in the intraooplasmic concentrations of second messengers such as cyclic adenosine 3′,5′‐ monophosphate and Ca2+ through their activity on cumulus cells (see Silvestre et al., this issue). This time‐sequence shows a representative fluorescent intensity profile of the Ca2+ wave triggered in cumulus cells by the addition of FSH in an immature bovine cumulusoocyte complex loaded with the fluorescent Ca2+ indicator Fluo‐4 FF/AM. The top image shows the cumulus‐oocyte complex at the time of FSH addition, and the bottom image shows the same cumulus‐oocyte complex 162 sec after exposure; intermediate images were taken at 48, 66, 84, 108, 132 sec from the addition of FSH, respectively.
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