Abstract

AbstractComputed cost function for in silico optimization of a cryoprotectant removal protocol. Karlsson et al. used membrane permeability data to predict the response of rhesus monkey oocytes to various methods for removing intracellular propylene glycol. The process parameter‐space is shown as a gray plane; the corresponding response surface represents the expected cytotoxicity, and vertical surfaces (green and violet) demarcate predicted regimes of deleterious osmotic shock. See the accompanying article by Karlsson et al. in this issue.

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