Abstract
Ghrelin is a 28 amino acid peptide endogenous ligand for growth hormone secretatgogue receptor (GHS-R) that functions to stimulate growth hormone, regulate inflammation, appetite and energy balance. However, the regulation of ghrelin expression by pro-inflammatory cytokines during inflammation process has never been investigated systematically. This study was carried out to investigate the effects of major pro-inflammatory cytokines such as TNF-α and IL-6 on ghrelin expression using AR42J rat pancreatic cell line as model system. The cells were treated with different concentrations of the cytokines for 24 hours. Real-Time RT-PCR, Western blot and densitometry analysis were carried out to quantify ghrelin mRNA and protein expression, respectively. Although TNF-α and IL-6 stimulation resulted in a general down-regulatory pattern in both mRNA and protein expression of ghrelin, stimulation with 5 ng/ml of TNF-α and IL-6 slightly induced the expression of ghrelin expression. However, higher doses of the cytokines ranging from 10-50 ng/ml suppressed the ghrelin expression in a dose-dependant manner. These results indicate that ghrelin in AR42J pancreatic cell line is regulated by the pro-inflammatory cytokines and that the dosages of the cytokines play an important factor in the regulation of the expression.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
