Abstract
Thuja orientalis a monoecious widely grown ornamental plant in Pakistan. T. orientalis plants were observed being severely attacked by Fusarium wilt during a survey of the landscape areas of District Faisalabad. Infected plants had severe symptoms of leaves wilting, chlorosis, and necrosis. Wilt disease-affected samples of Thuja orientalis were collected from the University of Agriculture, Faisalabad, Pakistan, in 2017. The associated pathogen was identified based on morphological characters and molecular characterization by using ITS primers (ITS1-F/ITS4) specific to ITS regions of ribosomal RNA (rRNA) of the fungus. The morphologically characterized fungal isolates were used in pathogenicity. In the pathogenicity test, inoculated plants were compared with control plants (sprayed with distilled water). After 3-4 weeks, inoculated plants showed typical chlorosis symptoms. Pathogenicity test proved the F. solani as pathogen of wilt disease on Thuja. Keywords: Thuja orientalis, Morpankh, Fusarium solani, Fusarium wilt, pathogenicity.
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