Molecular Prevalence of Mycoplasma capri in Thanjavur region

Abstract

Goat rearing has emerged as a significant resource in rural areas even under unfavorable environmental conditions. Goat population in India has grown over the past few decades and stands at 148.88 million during 2019 (Census, 2019) owing to their greater socio-economic relevance. Goats, while being generally resistant to diseases, are highly susceptible to respiratory diseases, which account for almost 50% mortality amongst them. Irrespective of the etiology, the infectious respiratory diseases of sheep and goats contribute to 5.6% of the total diseases of small ruminants and is responsible for around 28.7 % mortality. Pneumonia has been noticed as one of the most frequently encountered condition and is responsible for around 28.7% mortality. Amongst various infections, Mycoplasmosis is one of the most dreaded diseases of goats. The present study was undertaken to detect the Mycoplasma capri infection in cauvery delta region of Tamil Nadu. All the collected tissue materials were subjected to isolation and PCR assay with Mycoplasma group specific primers (GPO- 1 and MGSO) which yielded 715 bp product and Mycoplasma capri specific primers (P 4 and P 6) which gave an amplicons of 195 bp products. The findings indicate that the PCR assay is very simple and useful method for detecting the mycoplasma infection directly from the tissue materials in a very short span. Keywords: Mycoplasma capri, PCR, Infection, detection and tissue materials