Molecular phylogeny, origin and taxonomic implications of the tribe Cacteae (Cactaceae)

Abstract

This study aimed to test the phylogenetic relationships of the tribe Cacteae, the generic circumscription within the tribe, in particular, the monophyly of the genus Ferocactus, and to provide a biogeographical hypothesis about the origin of Cacteae. The analysis included 135 species from all of the 27 accepted genera and four outgroup species. Five chloroplast regions were sequenced, aligned, and coded postulating gaps, simple sequence repeats (SSRs), and inversions as potential synapomorphies, and their contributions to phylogenetic reconstruction were evaluated. The phylogenetic analyses recovered 63% of the genera as monophyletic. The contribution of rpl16, trnL-F and psbA to the phylogenetic signal was higher than in the two more slowly evolving genes (rbcL, matK), but the gaps and SSRs supported some of the genera. This result differs from those of previous phylogenetic studies in which less than 35% of the genera were recovered as monophyletic. In this work, Astrophytum and Echinocactus were re-circumscribed with five and four species, respectively. Turbinicarpus was found to be polyphyletic; 11 species correspond to Turbinicarpus s.str., whereas a highly supported clade corresponded to Rapicactus, and three species need further study. Contrary to its current circumscription, Ferocactus was not supported as monophyletic because it is polyphyletic concerning Glandulicactus, Leuchtenbergia, Stenocactus and Thelocactus. We recognize this group of genera as the Ferocactus clade in which the species share the presence of scales in the pericarpel and ribbed stems, whether tuberculated or not. The Cacteae seem to have originated in the Sierra Madre Oriental and then dispersed to the Mexican Plateau, where radiation and diversification occurred at the boundaries of the Miocene–Pliocene Epoch. The development of the Mexican Plateau and the Trans-Mexican Volcanic Belt may have favoured the isolation of the Cacteae. A taxonomic diagnosis is presented for the tribe Cacteae and 18 genera that we now recognize.