Molecular phylogenetic analysis of Pulmonata (Mollusca:Gastropoda) on the basis of Histone-3 gene

Vijaya Sai Ayyagari,Krupanidhi Sreerama

doi:10.1186/s43088-019-0014-z

Abstract

BackgroundIn the present study, phylogenetic relationships withinHeterobranchia in particular to Pulmonata were evaluated by means of Histone-3(H3) gene sequence information. H3 gene is a slow evolving marker and is usefulin resolving the deep level relationships. This is the first study to report thephylogeny of Pulmonata with more number of representatives from the group on thebasis of H3 gene.ResultsThe major groups within Heterobranchia viz. Lower Heterobranchia,Opisthobranchia, and Pulmonata were non-monophyletic. A few of the pulmonategroups’ viz. Planorbidae, Lymnaeidae, Siphonariidae, Veronicellidae, andStylommatophora were recovered as monophyletic. The concepts of Eupulmonata andGeophila were not observed in the present study.ConclusionsThe present study was undertaken with an objective to study thephylogeny of Pulmonata reconstructed on the basis of H3 gene and its ability toresolve the deeper divergences in Pulmonata. However, the resolution at thedeeper nodes is limited. There is a good resolution at the level of genera. Inthe future, inclusion of more number of taxa with increased sequence length ofH3 marker may yield resolved topologies that may shed more insights into thephylogeny of Pulmonata.

Highlights

In the present study, phylogenetic relationships within Heterobranchia in particular to Pulmonata were evaluated by means of Histone-3 (H3) gene sequence information
The three species of gastropods were so far not sequenced for H3 gene and the analyzed sequences of these three taxa were deposited for the first time to the National Centre for Biotechnology Information (NCBI) and DNA Databank of Japan (DDBJ)
3.4 Pulmonata Siphonariidae: Siphonaria concinna and Siphonaria denticulata formed a monophyletic clade in Bayesian inference (BI) (0.94) and maximum likelihood (ML) analyses

Summary

Introduction

Phylogenetic relationships within Heterobranchia in particular to Pulmonata were evaluated by means of Histone-3 (H3) gene sequence information. H3 gene is a slow evolving marker and is useful in resolving the deep level relationships This is the first study to report the phylogeny of Pulmonata with more number of representatives from the group on the basis of H3 gene. The word “Euthyneura” was coined by Spengel [3] for uniting the taxa that reverted from the crossed state of visceral loop to uncross state during the process of detorsion This is not considered as a synapomorphic character for “Euthyneura” due to the presence of this condition in few “Streptonerous” taxa [1]. Euthyneura is further divided into Opisthobranchia and Pulmonata Each of these two groups is distinct on the basis of several synapomorphic characters of the nervous system [2]. Different molecular phylogenetic studies have yielded Lower

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