Molecular Phylogenetic Analysis Of Morkaraman Sheeps In Bingol Region

Abstract

In the present study, we report mitochondrial DNA (mtDNA) 16S rRNA gene sequence analysis of the Morkaraman ewe, (Ovis aries) was investigated. In domestic sheep, the 16S rRNA gene is ca 1574 bp in length. Partial fragment of 536 bp 16S rRNA gene of mtDNA was amplified by using designed primers from published data of Sheep sequences derived from National Center for Biotechnology Information (NCBI) web server (ncbi.nlm.nih.gov/NC_001941.1). Homology of the sequences obtained from the present work were performed by using BLAST program at NCBI. Obtained 16S rRNA gene sequences were submited to the GenBank database with accession numbers, KU686952.1, KU686953.1, KU686954.1, KU686955.1, KU686956.1, KU686957.1, KU686958.1, KU686959.1, KU686960.1, KU686961.1 and KU686962.1, respectively. According to nucleotide differences of mtDNA 16S rRNA gene region 10 polymorphic regions and 8 haplotypes were detected in Turkish domestic sheep Morkaraman (n=11). The nucleotide composition averages of all the sequences was 32.1% Adenine (A), 24.2% Thymine (T), 22.2% Cytosine (C) and 21.5% Guanine (G); G+C was 43.7%. DNA polymorphism based on 16S rRNA gene sequences, haplotype diversity (Hd) and nucleotide diversity (π) were found to be 0,9273±0,00442, and 0,00427±0,00125, respectively. Based on gene sequences information, in sheeps, mtDNA haplotypes, the phylogenetic relationship among mtDNA polymorphism, haplotypes and relationship between native sheep breeds and foreign sheep breeds were determined and discussed. Thus, sequence analysis of mitochondrial 16S rRNA gene can be used as a tool for phylogenetic analysis of Morkaraman sheep.