Abstract
Molecular biological methods have been used to study pituitary disorders in recent years. Techniques, such as Southern and Northern hybridization, in situ hybridization (ISH), polymerase chain reaction (PCR), single-strand confirmation polymorphism (SSCP) analysis, and clonal analysis, have been used to examine normal and neoplastic pituitary tissues. These methods have contributed greatly to advances in the understanding of pituitary disorders. This chapter will review some basic molecular biological techniques and illustrate their applications to the study of pituitary disorders.
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