Abstract
e21043 Background: In this study, desorption electrospray ionization (DESI) mass spectrometry (MS) was used in an imaging mode to interrogate the lipid profiles of thin tissue sections of human transitional cell carcinoma (TCC) of the urinary bladder and adenocarcinoma of the prostate to determine whether DESI-MS could discriminate between cancerous and noncancerous tissues. Methods: DESI-MS images showing the spatial distributions of particular glycerophospholipids (GPs) and free fatty acids in the ion mode were compared to serial tissue sections that were stained with hematoxylin and eosin (H&E) among 19 matched pairs of cancerous and noncancerous bladder tissues and among 34 pairs of tissue from prostate cancer patients. Increased absolute and relative intensities were utilized to assess for particular compounds in the tumor regions of the samples. Principal component analysis (PCA) was used to generate unsupervised statistical images from the negative ion mode data and which were used to assess for ag...
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