Molecular organization and chromosomal localization of six highly repeated DNA families in the bovine genome

Abstract

Abstract The genomic structure and cytogenetic position of five localized (satellite) and one dispersed highly repeated bovine DNA families were determined using Southern blotting and fluorescence in situ hybridization. Oligonucleotides were synthesized to published sequence data for all six repeats and either used directly as hybridization probes (1.711a‐Sau, 1.715, bovine‐Pst) or as primers in PCR in order to obtain longer‐sized probes (1.709, 1.711a‐insertion, 1.711b‐insertion). Results from Southern hybridization following digestion with 26 restriction enzymes revealed type A ladder patterns for all satellites, with evidence for novel subdomains demonstrated in the 1.709, 1.715 and 1.711b families. In situ hybridization localized all five satellite repeats to the centromeric regions of many (e.g., 1.709) or all (e.g., 1.715) autosomes. In addition, three of the satellites also hybridized interstitially. The dispersed bovine‐Pst element hybridized intensely along all chromosomes except at autosomal cen...