Molecular mechanisms of regulation of anthocyanine biosynthesis, photosynthesis, and frost resistance of winter wheat seedlings treated with the 5-aminolevulic acid

Abstract

It was found that in anthocyanin-enriched coleoptiles of winter wheat EtW5 variety, the exogenous 5-aminolevulinic acid (ALA) increased the content of anthocyanins by a factor of 1.5 and the expression level of structural (PAL, CHS) and regulatory (PAP-1) genes of the anthocyanin biosynthesis pathway. In coleoptiles of Vladi plants containing 33 times less anthocyanins compared to EtW5, the expression of CHS and PAP-1 was reduced and additionally inhibited by ALA. Thus, the genetic activity of the phenylpropanoid and flavonoid sites of the anthocyanin biosynthesis pathway shows distinct variety specificity and a dependence on the level of anthocyanins. ALA significantly reduced the thermal dissipation of excitation energy in PSII complexes in the EtW5 variety enriched with anthocyanins and significantly increased the level of these processes in the Vladi variety. Variety-specificity was noted in the levels of frost resistance of two varieties – high (88 % of surviving plants exposed to a temperature of –8 °С for 5 hours) in the EtW5 variety and lower (80 %) in the Vladi variety. ALA increased the frost resistance of both varieties due to an increase in the content of anthocyanins and proline in the coleoptiles of the EtW5 variety and a higher proline content in the Vladi variety.