Abstract
Photosynthesis is arguably the most important biological process,by it organisms harvest solar energy and transfer it into chemical energy for growth and reproduction.The photosynthetic bacteria are the earliest microbe with photosynthesis found on earth.The photosynthetic apparatus of purple bacteria is a nanometric assembly in the intracytoplasmic membranes and consists of pigment-protein complexes,the photosynthetic RC(Reaction center) and LH(Light harvesting).The primary processes of photosynthesis involve absorption of photons by LH complexes,transfer of excitation energy from the LH complexes to the photosynthetic RC,where the primary energy conversion takes place.The researches on molecular structure and mechanism of purple photosynthetic bacteria harvesting solar energy were summarized.Molecular biology techniques and spectroscopic analysis were applied to research the expression and function of puc2BA and pucsBA by the authors,and it was concluded that the puc2BA gene was normally expressed in Rhodobacter sphaeroides,the pucsBA gene of Rhodovulum sulfidophilum and the pufBA gene of R.sphaeroides were expressed in R.sphaeroides,and the heterologous LHII and native LHI were produced and assembled in the membrane.
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