Abstract
Objective To investigate the expression of microRNA (miRNA)- 145 and protein kinase Bγ (Akt3) in thyroid carcinoma and the molecular mechanism of miRNA-145 targeting Akt3 inhibiting the proliferation and invasion of thyroid carcinoma cells. Methods Real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) was used to detect the expression of microRNA-145 and Akt3 in 49 fresh thyroid carcinoma tissues and adjacent tissues. The relationship between the expression of microRNA-145 and Akt3 was analyzed. TPC-1 thyroid cancer cell line was used as the research object. The effects of microwave irradiation on the growth, invasion and metastasis of thyroid cancer cells were detected by methyl thiazol tetrazolium (MTT) and Transwell invasion assays. The expression levels of microwave irradiation-145 and Akt3 were detected by Western blotting. Results The results of FQ-PCR showed that the relative expression levels of miRNA-145 in thyroid carcinoma and adjacent tissues were 1.65±0.33 and 3.13±0.53, and those of Akt3 were 2.96±0.85 and 0.83±0.32, respectively. The difference was statistically significant (P=0.000). The relative expression levels of microRNAs-145 in TPC-1 cells and primary thyroid follicular cells were 1.91±0.57 and 3.47±0.59, and those of Akt3 were 3.62±1.04 and 0.76±0.32, respectively. There was statistically significant difference (P=0.000). The increase of A570 in TPC-1 cells transfected with anti-miR-145 was the fastest, and that in TPC-1 cells transfected with analogues of miR-145 was the slowest (P=0.000). As compared with TPC-1 cells without transfection and TPC-1 cells transfected only with liposome 2000, the number of invasive cells transfected with anti-miR-145 increased significantly (P=0.000), while the number of invasive cells transfected with micR-145 analogues decreased significantly (P=0.000). The expression levels of p-Akt and Akt-3 protein in miR-145-mimics-transfected and non-transfected TPC-1 cells were significantly lower than those in TPC-1 cells transfected with anti-miR-145. Conclusion MiR-145 can target down-regulate the expression of Akt3 to inhibit the proliferation and invasion of thyroid cancer cells. Key words: Thyroid cancer; MicroRNA-145; Protein kinase Bγ; Proliferation; Metastasis
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