Abstract
Tomato is infected by more than 100 pathogens that affect its production; therefore, it is crucial that commercial varieties integrate resistance genes. In this context, molecular markers improve the efficiency of the selection process. This study aimed to validate the efficacy of molecular markers in identifying genes resistant to six pathogens: Meloidogyne sp. (Mi-1 and Mi-1.2), Fusarium oxysporum f. sp. lycopersici (I-1 and I-2), Stemphyllium sp. (Sm), Phytophthora infestans (Ph3), tomato yellow leaf curl virus (Ty-2 and Ty-3) and spotted wilt virus (Sw5b). Protocols for 10 pairs of markers associated with resistance genes were adapted and tested on 20 genotypes. Pathogen resistance genes were identified in 17 advanced lines and three commercial hybrids of tomato. Molecular markers distinguished lines with resistance genes to six important diseases in tomato cultivation, which could therefore be used for the development of new varieties.
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