Molecular Markers Linked with Sterility Mosaic Resistance in Pigeonpea (Cajanus cajan L. Millsp.)

Abstract

Background: Pigeonpea is an important protein-rich legume and one of the potential crops for rainfed agriculture in many parts of the semi-arid tropics. Worldwide, India ranks first both in area and production. Despite that, the productivity levels in India are low because of various biotic and abiotic stresses, out of which Sterility mosaic disease (SMD), which is caused by Pigeonpea Sterility Mosaic Virus (PPSMV), is a severe constraint during crop production. In the context of the above facts, resistant breeding is one of the most economical and eco-friendly approaches. The present investigation aimed to identify microsatellite markers linked to SMD resistance and potential resistant genetic resources. In this field- laboratory investigation carried out during 2017-2018, thirty genotypes and susceptible check ICP 8863 were evaluated in a randomized block design (RBD) with two replications in SMD sick plots. Observations were recorded based on visual symptoms for SMD incidence, and percent disease incidence (PDI) was calculated. In addition, a molecular study for the same genotypes was carried out with a total of 20 SSR markers. Out of 20 SSR primers, only 16 were amplified, from which only a single SSR marker (AHSSR20) showed polymorphism while the remaining 15 were monomorphic. The marker AHSSR20 have the potential to discriminate resistant and susceptible genotypes. AHSSR 20 primer assign 160 bp bands in resistant genotypes viz., BDN-711, PT-04-281, and PT-704-1-2 while ever, 172 bp bands assign in susceptible genotypes viz. ICP-8863, PT-04-378, PT-012-20, and PT-016-3. Accordingly, we conclude that AHSSR20 is a potent marker that can be helpful to discriminate between susceptible and resistant genotypes and can be used for direct selection of resistant genotypes during early segregating generation and marker-assisted breeding.