Abstract
Kentucky bluegrass (Poa pratensis L.) is a highly adapted and important turfgrass species in cool-season climates. It has high and variable polyploidy, small and metacentric chromosomes, and a facultative apomictic breeding system. As a result of the polyploidy and apomixis, identifying hybrids for Mendelian selection, identifying fixed apomictic progeny of desirable hybridizations for cultivar development, or differentiating among cultivars with subtle phenotypic differences is challenging without the assistance of molecular markers. Herein, we show data and review previous research showing the uses and limitations of using molecular markers for hybrid detection, apomixis assessment, and cultivar discrimination. In order to differentiate among different apomictic offtypes, both molecular markers and flow cytometry are necessary. For assessing similarity among progeny of hybridizations, as well as discriminating among cultivars, sets of markers are necessary and cryptic molecular variation must be considered. High throughput genotyping platforms are critical for increased genotyping efficiency.
Highlights
The Poa genus comprises approximately 500 species, depending on taxonomic sources [1]
Five microliters of Chicken Erythrocyte Nuclei (CEN; BioSure) were spiked in as an internal standard, and samples were incubated at room temperature in the dark for
Typical are shown in Figure 1. and not all markers both parents are expected due progeny, to meiotic segregation andmarkers assortment of parental
Summary
The Poa genus comprises approximately 500 species, depending on taxonomic sources [1]. It is a highly adaptable genus, spanning all continents and diverse habitats. There are caespitose species used for rangeland restoration, such as P. secunda [2] and P. densa [3]. There are weedy species like P. annua, whose presence is so pervasive that it is begrudgingly used in lawns and golf courses [4]. Other species have limited or strategic value as turfgrasses, such as P. trivialis [5] and P. arachnifera [6]. The most important agronomic species in the genus is P. pratensis, commonly called Kentucky bluegrass in the USA and smooth-stalked meadowgrass in Europe
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