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Abstract
Tomato mosaic virus (ToMV) is one of the most infectious virus diseases in tomato (Solanum lycopersicum L). The practical and effective method of controlling this disease is through genetic control by using major resistance genes. So far, three genes Tm-1, Tm-2 and Tm-22 conferring resistance to ToMV have been reported and utilized in tomato culti-var development. Marker assisted selection (MAS) has become very important and useful tool in selection of ToMV re-sistant tomato lines or hybrids. The objective of this research was to identify allele-specific PCR-based, cleaved ampli-fied polymorphic sequence (CAPS), and allele-derived single nucleotide polymorphism (SNP) markers for Tm-2 loci. Four allele-specific PCR-based markers were identified: one for Tm-2, one for Tm-22, and two for the susceptible allele tm-2. Three allele-derived CAPS markers were identified, which can identify and distinguish three alleles, tm-2, Tm-2 and Tm-22 in tomato germplasm. Three SNP markers were developed specific for Tm-2 locus. These markers will pro-vide breeders with a tool in selection of Tm-2 and Tm-22 resistance genes in tomato breeding program.
Highlights
Tomato mosaic virus (ToMV) is one of the most infectious virus diseases in tomato (Solanum lycopersicum L. formerly, Lycopersicon esculentum Mill)
Indicating the two primers can be used as PCR-based markers to select the susceptible allele tm-2 in tomato
The primer pair Tm2R-f1c/ Tm2R-r4 amplified the DNA from tomato genotypes containing either Tm-2 or Tm-22 with homozygous or heterozygous condition, indicating the primer pair can be used to select either Tm-2 or Tm-22 gene (Table 1)
Summary
Tomato mosaic virus (ToMV) is one of the most infectious virus diseases in tomato (Solanum lycopersicum L. formerly, Lycopersicon esculentum Mill). Three genes, Tm-1, Tm-2 and Tm-22 of ToMV resistance have been reported and used in tomato cultivar development [1,2,3,4,5]. The Tm-1 gene was introgressed into S. lycopersicum from the wild tomato species S. habrochaites and mapped to chromosome 5 and it conferred resistance to ToMV strains 0 and 2 [1,4,6,7]. The other two genes Tm-2 and Tm-22 were introgressed from S. peruvianum and were located closely to the centromere of chromosome 9 and are considered to be allelic and Tm-2 conferred resistance to ToMV strains 0 and 1, whereas allele Tm-22 conferred resistance to
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