Abstract
Santa Rosa and Frontier are the major Japanese plum (Prunus salicina Lindl.) cultivars grown throughout the world. The present investigation was performed to understand the genetic relatedness among in vitro propagated plum cultivars Santa Rosa and Frontier using PCR based molecular markers. For the study, three arbitrary markers viz. RAPD (Random amplified Polymorphic DNA), ISSR (Inter-Simple Sequence Repeats) and SCoT (Start Codon Targeted) were used. In RAPD analysis, 18 primers out of 28 amplified and generated 33 scorable bands. The allelic variations when analysed, revealed 84% similarity between these two cultivars with highest polymorphic information content of 0.78. Similarly, 15 ISSR primers produced 73 amplicons with an average of 4.86 amplicon per primer and similarity coefficient ranging from 62 to 67%. Seven SCoT primers out of 26 resulted in a total of twenty- six scorable bands with 24 polymorphic bands. Cluster analysis from all the three markers used broadly divided plum cultivars Santa Rosa and Frontier into two major clusters containing in vitro shoots, their progenies and mother trees of respective genotypes. The study concluded that these three marker systems were found to be effective in revealing genetic relationship of these two commercially important plum cultivars.
Highlights
Plums are widely grown in most of the temperate regions of the world
In this study resolving power of RAPD, ISSR and SCoT was used to confirm the genetic relatedness in Plum cvs
For RAPD analysis eighteen RAPD primers out of 28 resulted in 33 scorable bands ranging from minimum of 1 in primers OPA 08, OPB 01, OPB 02, OPB 06, OPB 07, OPB 08, OPB 10, OPB 14, OPE 04 and OPE 14 to a maximum of 5 in OPC 02
Summary
Plums are widely grown in most of the temperate regions of the world. Plum belongs to family Rosaceae, genus Prunus and subgenus Prunophora. Japanese plum is a dominant species with broad adaptation from temperate to subtropical regions of the world. Santa Rosa and Frontier are two prime cultivars of Japanese plum planted commercially in India (Thakur et al, 2020) whose genetic relationship is not known. Santa Rosa and Frontier using RAPD, ISSR & SCoT markers (Thakur et al, 2018; Thakur et al, 2020; Thakur et al, 2021). The outcome of these studies was used to elucidate genetic relatedness between these two commercial plum cultivars for their future use in breeding for the development of new cultivar
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