Abstract
Late blight (LB), caused by the oomycete Phytophthora infestans, is one of the most devastating diseases of tomato. Three major genes Ph-1, Ph-2 and Ph-3 conferring resistance to LB have been identified and mapped to the chromosomes 7, 10 and 9, respectively. However, PCR-based molecular markers associated with these genes are limited. Molecular markers are extremely useful in the screening and selection of tomato lines for the development of LB resistant genotypes. The objective of this study was to identify molecular markers associated with Ph-3 gene conferring LB resistance in tomato. Four co-dominant markers were found to be associated with Ph-3, all of which were sequence characterized amplified region (SCAR) type. Breeding lines and cultivars were inoculated with a field isolate of Phytophthora infestans to collect phenotypic data on disease resistance. Genotypic data from molecular markers associated with Ph-3 were in close agreement with the phenotypic data for the lines tested. With the verification of genotypic data from novel molecular markers in known genotypes supported by phenotypic data, the novel molecular markers may be useful in screening tomato populations aiming to develop LB resistant genotypes or cloning the LB resistant genes.
Highlights
Late blight (LB), caused by the oomycete Phytophthora infestans (Montagne, Bary), is one of the most poten-How to cite this paper: Panthee, D.R., Gardner, R.G., Ibrahem, R. and Anderson, C. (2015) Molecular Markers Associated with Ph-3 Gene Conferring Late Blight Resistance in Tomato
Genetic resistance to LB in tomato has been of interest for many years, and three major resistance genes have been identified in the red-fruited tomato wild species S. pimpinellifolium, including Ph-1, Ph-2 and Ph-3, which have been mapped to tomato chromosomes 7, 10, and 9, respectively
Four sequence characterized amplified region (SCAR) molecular markers were found to be associated with Ph-3 gene
Summary
Late blight (LB), caused by the oomycete Phytophthora infestans (Montagne, Bary), is one of the most poten-How to cite this paper: Panthee, D.R., Gardner, R.G., Ibrahem, R. and Anderson, C. (2015) Molecular Markers Associated with Ph-3 Gene Conferring Late Blight Resistance in Tomato. (2015) Molecular Markers Associated with Ph-3 Gene Conferring Late Blight Resistance in Tomato. The resistance conditioned by Ph-2, a single incomplete-dominant gene mapped to the lower end of the long arm of tomato chromosome 10 [2], provides partial resistance to several isolates of race T-1 [1] [3]. LA 1269 or PI365957) at the Asian Vegetable Research and Development Center (AVRDC) in Taiwan [4] This gene is much more useful than Ph-1 and Ph-2 and confers incompletely dominant resistance to a wide range of P. infestans isolates of tomato, including those that overcome Ph-1 and Ph-2 [6]. Comm.), have succeeded in transferring LB resistance genes to fresh-market and/or processing tomato breeding lines or hybrid cultivars using a combination of phenotypic screening and MAS. The objective of this study was to identify molecular markers associated with Ph-3 gene conferring LB resistance in tomato
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