Molecular marker for characterization of traditional and hybrid derivatives of Eleusine coracana (L.) using ISSR marker

Jayalakshmi Venkatesan,Vasuki Ramu,Thilaga Sethuraman,Chandrasekaran Sivagnanam,Ganesh Doss

doi:10.1186/s43141-021-00277-1

Jayalakshmi Venkatesan, Vasuki Ramu + Show 3 more

Open Access

https://doi.org/10.1186/s43141-021-00277-1

Copy DOI

Abstract

BackgroundFinger millet is the most important food grain in the world for its nutritional benefits. Finger millet is genetically and geographically diverse and widely spread in the African and Asian sub-continent. Therefore, the present study was undertaken to analyze the genetic diversity using ISSR genetic markers using 15 ISSR primers. ResultsAbout 23 genotypes of widely cultivated finger millet cultivars of economically important ones were characterized and the ISSR markers were critically analyzed for their performance with parameters such as polymorphic information content (PIC), effective multiplex ratio (EMR), marker index (MI), and resolving power (RP). In this study, 175 loci were scored across the 23 cultivars of finger millet, and out of these 173 loci (98%) were polymorphic, revealing the suitability of these loci for genetic diversity analysis with ISSR marker. The average number of polymorphic loci per primer was 11.50 with varying sizes from 100 bp to 2500 bp. ISSR primers that showed higher polymorphism were found to have higher EMR and MI values up to 15.30 and 13.44, respectively. ConclusionHigh degree of polymorphism supported with distinct differences of all the marker parameters revealed the suitability of ISSR markers for determining the genotypic differences based on ISSR markers among the 23 genotypes of finger millet. The possible application of the ISSR marker in the conservation and management of finger millet genetic resources is discussed.

Highlights

Finger millet is the most important food grain in the world for its nutritional benefits
Based on the polymorphic information, parameters such as polymorphic information content (PIC), effective multiplex ratio (EMR), marker index (MI), and resolving power (RP) were critically analyzed for utilizing these Inter Simple Sequence Repeat (ISSR) primers for genetic variability as well as to develop cultivar-specific ISSR markers for germplasm management
In this study, we tested with 6 traditional varieties from Tamil Nadu, 10 traditional varieties from Karnataka, and 7 hybrid varieties from Karnataka even though the samples were collected from different regions but some were having a close relationship between them was observed in the dendrogram

Summary

Introduction

Finger millet is the most important food grain in the world for its nutritional benefits. Finger millet is genetically and geographically diverse and widely spread in the African and Asian sub-continent. Finger millet is one of the most important food crops in Africa and Asia [1]. Finger millet occupies 12% in more than 25 countries in Asia and Africa [2]. Finger millet germplasm and its derivatives are said to be extremely tolerant to drought and salt stress [3]. Finger millet is well known to be one of the potential crops to alleviate the problem of nutritional deficiency being faced worldwide [4]. Considering the importance of Understanding the genetic diversity of finger millet is one of the vital requirements for crop improvement [8]. Genetic distance or genetic similarity are the most important parameters employed to calculate the genetic

Methods

Results

Discussion

Conclusion