Molecular marker assisted gene stacking for disease resistance and quality genes in the dwarf mutant of an elite common wheat cultivar Xiaoyan22

Weijun Zheng,Yanru Feng,Song Li,Qi Zhou,Zihui Liu,Shoucheng Chai

doi:10.1186/s12863-020-00854-2

Weijun Zheng, Yanru Feng + Show 4 more

Open Access

https://doi.org/10.1186/s12863-020-00854-2

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Journal: BMC genetics	Publication Date: Apr 23, 2020
Citations: 14	License type: open-access

Affiliation: North West Agriculture and Forestry University

Abstract

BackgroundDevelopment of wheat cultivars with multiple disease resistance and high quality are major objectives in modern wheat breeding programs. Gene stacking is an efficient approach to achieve this target. In this study, we pyramided yellow rust resistance gene (Yr26), powdery mildew resistance gene (ML91260) and high-molecular-weight glutenin subunits Dx5 + Dy10 into the dwarf mutant of an elite wheat cultivar, Xiaoyan22.ResultsSix pyramided wheat lines were obtained by molecular marker-assisted selection (MAS) and field evaluation of disease resistance. The desirable agronomic traits of pyramided lines, their identity with the original cultivar Xiaoyan22 except for plant height, tiller number and disease resistance, was achieved in this study. Meanwhile, the yield of pyramided lines is higher than Xiaoyan22 in the field test. In addition, analysis of flour quality indicated that the dough stability time of pyramided lines was longer than that of Xiaoyan22.ConclusionsSix pyramided wheat lines with two disease resistance and high quality were achieved in this study. It is feasible to improve multiple agronomic traits simultaneously by rational application of MAS.

Highlights

Development of wheat cultivars with multiple disease resistance and high quality are major objectives in modern wheat breeding programs
PyramidingPM/Yellow rust (YR) resistance genes and HWM-GS by marker-assisted selection (MAS) With appropriate DNA markers for yellow rust and powdery mildew resistance and HWM-GSDx5 + Dy10, marker selection was exercised in each generation from Double -cross F1 hybrid (DCHF1) to TCHF1BC2F2 (Table S1)
Foreground selection was exercised in TCHF1BC1 to TCHF1BC2 to select plants that carried all genes in heterozygous condition; 11 plants were selected and used to raise TCHF1BC2F1

Summary

Introduction

Development of wheat cultivars with multiple disease resistance and high quality are major objectives in modern wheat breeding programs. We pyramided yellow rust resistance gene (Yr26), powdery mildew resistance gene (ML91260) and highmolecular-weight glutenin subunits Dx5 + Dy10 into the dwarf mutant of an elite wheat cultivar, Xiaoyan. The alleles of the highmolecular-weight glutenin subunit gene determines the processing quality of wheat in most cases. The main cultivated varieties of wheat in Huang-Huai are medium-gluten wheat, and the wheat cultivars with strong gluten are still deficient This indicates that wheat quality must be improved along with improving PM and YR resistance. Eight QTLs/genes for seven different traits were pyramided into a variety PBW343, and the new pyramiding lines (PYLs) were resistant against three rust pathogens, and the grain quality was improved [12]. Bacterial blight resistance genes Xa13 and Xa21, were introgressed into indica rice cultivar PR106 by MAS; those two genes provided durable blight resistance to Indian Rice Variety MTU1010 [14]

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Conclusion