Abstract
The study was aimed to develop rust resistant genotypes with genome of prominent wheat variety HS240 by pyramiding of linked leaf rust and stem rust resistance genes Lr19 and Sr25 with stripe rust resistance gene Yr15. The genotypes FLW13 and FLW20 were used as gene donors for transfer of Yr15 and Lr19/Sr25, respectively. The molecular markers scs265, wmc221, PSY1-E1, and Gb linked to Lr19/Sr25 and barc8 and gwm11 linked to Yr15 were used for foreground selection. Background selection, involving 58 simple sequence repeat (SSR) markers polymorphic between HS240 and FLW20; 72 between HS240 and FLW13 was carried out to recover the genome of HS240 in advanced bulks derived from a cross HS240*2/FLW20//HS240*2/FLW13. The SSR based genome recovery in selected advanced breeding lines, WBM3682 and WBM3684 was 90.8% and 93.3%, respectively. The 35 K SNP array based analysis of genomic regions of HS240 also substantiated the results of genomic recovery estimated through SSR markers. The selected line WBM3682 recorded average grain yield of 25.7q/ha and showed its superiority over parental check HS240 under rainfed situations. This study has led to development of rust resistant wheat carrying genome of HS240.
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