Molecular mapping of a new red mutant gene (Rs) in upland cotton

Abstract

In recent years, there has been slow progress in improving cotton yield. It is known that the F1 generation from the cross of the new red mutant and the normal green leaf plant has high photosynthetic efficiency. Therefore, cloning the new red mutant gene and further introducing it into other crops through transgenic techniques is a promising approach for achieving high photosynthetic efficiency through breeding. To map this new mutant gene, tentatively named Rs, the authors constructed an F2 generation containing 1214 individual plants from mutant EH083 (Gossypium hirsutum) and Hai 7124 (Gossypium barbadense). Fifty-five pairs of simple sequence repeats and sequence-related amplified polymorphism (SRAP) primers on chromosome 7 were selected to screen the two parents. Finally, the Rs gene was mapped at the 0.3 cM interval flanked by markers NAU3735 and NAU1048.