Molecular Mapping and Marker Assisted Selection of Soybean Mosaic Virus Resistance Gene RSC12 in Soybean

Abstract

The P 1 , P 2 , F 1 plants, F 2 population and F 2:3 lines from the cross of Qihuang22×Nannong1138-2 were inoculated with the soybean mosaic virus (SMV) strain SC12 for identification of their resistance in the greenhouse. Qihuang22 and F 1 individuals were resistant(R), and Nannong1138-2 were susceptible(S). The F 2 population segregated in a ratio of 3(R):1(S), and the F 2:3 lines exhibited a segregation pattern of 1(R):2(Segregating):1(S). These results indicated that a single dominant gene controlled the resistance to SC12. A F 2 population of Qihuang22×Nannong 1138-2 with 219 individuals was constructed for molecular mapping of resistance gene RSC12 to soybean mosaic virus in soybean. Linkage analysis with bulk segregant analysis (BSA) demonstrated that the resistance gene RSC12 was located on the linkage group F and linked with seven SSR markers. The order and genetic distance of markers linked with RSC12 were Sat_297 6.4 cM Sat_234 4.9 cM Sat_154 1.1 cM Satt114 0.7 cM SOYHSP176 1.6 cM Satt334 2.4 cM RSC12 6.3cM Sct_033. The marker-assisted selection (MAS) efficiency of SSR markers Satt334 and Sct_033 was evaluated in F 2 , F 3 and F 4 populations. The results showed that the MAS efficiency of Satt334 and Sct_033 was more than 85%, and that the MAS efficiency reached as high as 95% when these two markers were co-used. Therefore, the two SSR markers can be used effectively in selecting for resistance genes RSC12 instead of inoculation identification.