Molecular-Level Dengue Fever Diagnostics: Developing a Combination of RT-LAMP and Paper-Based Devices

Abstract

This article describes the development of an inexpensive but robust and easy-to-use point-of-care diagnostic device that can be used for the detection of dengue fever at the molecular level (i.e., the detection of dengue virus using a piece of paper). To date, the clinical diagnosis of dengue fever mainly relies on enzyme-linked immunosorbent assay-based examinations for a specific antigen. However, protein-based diagnostics are used at a relatively late stage of the infection, and there is a need to develop a simple and low-cost diagnostic device for the detection of dengue fever at the early stages of the infection.