Abstract
This study aims to isolate and recognize (native/indigenous) hydrocarbon degrading fungal strains from oil-laden soils, at different geographical locations in KRI oilfields, Iraq. The identification was mainly based on macroscopic and microscopic examinations, but due to morphological similarities, which make the identification at species level doubtful in some cases, an attempt was made to use molecular techniques as well, as confirmatory/comparative analysis. The extracted DNA from hypha was used for polymerase chain reaction (PCR) amplification, using specific internal transcribed spacer primer (ITS1/ITS2). PCR product sequencing analyses were compared with the other related sequences in GenBank (NCBI) for molecular evolutionary analyses. Results showed that; out of 68 screened fungal strains; only two genera (i.e. Aspergillus spp. and Penicillium sp.) were identified as the most potent hydrocarbon degrading fungi. DNA sequence analysis confirmed the validity of the species as; Aspergillus fumigatus-KU321562.1; A. flavus-MH270609.1; A. niger-MK452260.1 and Penicillium chrysogenum-MK696383.1.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
